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CEM 832 - Mass Spectrometry - Spring 2014
Department of Chemistry
Michigan State University

Course Outline


Introduction

Resources

Reid Research Group 

Chemistry Dept. Home Page

MSU Home Page






Required Text: J. Throck Watson and O. David Sparkman. “Introduction to Mass Spectrometry: Instrumentation, Applications, and Strategies for Data Interpretation”, 4th Edition, Wiley, 2007.


 

 


The course material will be selected from the following topics:

 

Part I - General Principles and Historical Overview

 

1.    General Principles

 

2.    Historical Overview of Mass Spectrometry

 

3.    Definition of Mass Spectrometry Terms

 

             Mass-to-charge ratio (m/z)

             Resolution

             Monoisotopic mass versus average mass

             Mass accuracy

 

Part II - Mass Spectrometry Instrumentation

 

1.          Vacuum Systems:

 

Ion Free Path

Common Units of Pressure

 

2.          Sample Introduction Methods:

 

Direct Vapor/Batch Inlets

Direct Insertion Probe

Gas Chromatography

Direct Ionization of Thermally Labile Sample

 

3.      Detection Methods:

 

4.          Data Acquisition:

 

5.      Ionization Methods:

             

Electron Ionization (EI)
Chemical Ionization (CI)
Inductively Coupled Plasma (ICP)
Fast Atom Bombardment (FAB)
Matrix-Assisted Laser Desorption/Ionization (MALDI)
Electrospray Ionization (ESI)

 

6.      Mass Analyzers and Fundamentals of Ion Motion:

          

Magnetic and Electric Sector

Time-Of-Flight

Quadrupole

Quadrupole Ion Trap

Fourier Transform Ion Cyclotron Resonance

Orbitrap

 

6.      Tandem Mass Spectrometry:

 

Triple Quadrupole

Quadrupole Ion Trap

Hybrid Quadrupole/Ion Trap (e.g., coupled with TOF, FT-ICR, Orbitrap)

7.      Emerging Mass Spectrometry Methods: (as time permits)

 

e.g., Imaging Mass Spectrometry, Ion Mobility

 

Part III - Gas-Phase Fragmentation Reactions

 

Part IV - Practical Structure Elucidation of Biologically Derived Ions

 

1.            Peptides and Proteins

 

2.            Metabolites and Lipids

 

 

This page maintained by Gavin Reid. Last Updated: January 4th, 2010