Chemistry 434

Fall 2017

Advanced Analytical Chemistry - Instrumental Methods of Analysis



Course Organization and Other Information



Monday, Wednesday, Friday 1:50 to 2:40 pm




136 CEM




Friday, 9:10-10:00 AM,  183 CEM

Friday, 3:00-3:50 PM,  136 CEM


Course Website:

(complete course syllabus, course information and materials available via login on D2L)


Required Text: 

R. M. Granger, H. M. Yoghum, J. N. Granger and K. D. Sienerth, Instrumental Analysis, 1st Edition, Oxford University Press, 2017.



Professor Greg M. Swain

314 Chemistry Building

Tel. 355-9715 x229



Instructor Office Hour:  

Wednesday, 1:00 to 1:45 pm, 314 Chemistry, or by appointment.






TA Office Hour:   




Course Description

This in-depth course covers the design, operational principles and practical application of modern instrumental methods used in chemical analysis. These methods are used in the separation, identification and quantification of the chemical components of natural and artificial materials. Using a combination of problem-based learning approaches, case studies and traditional lectures, the student will develop critical thinking skills in the areas of instrument selection, method development and data interpretation.


 Course Objectives

1.     Understand the basic design and operating principles of some modern instruments used in chemical analysis, specifically separations, optical spectroscopy, electroanalytical methods, material characterization and surface analysis techniques, and analytical mass spectrometry.


2.     Understand the basics of experimental design and the use of statistical analysis to evaluate measurement data.


3.     Understand how to design experiments using these instruments to solve problems in chemical analysis.


4.     Learn how to use databases to search the scientific literature and how to read a scientific paper.


5.     Improvement of written communication skills through the preparation of a literature-based research paper on a current topic in analytical chemistry.


6.     Improvement of oral communication skills through delivering an oral presentation, as part of a team, on an assigned case study.



Course Organization

Section A: Foundations (Analytical Chemist’s Toolbox)

Chapter 1 – Method selection, detection figures of merit and response calibration

Chapter 5 – Signals and noise, and signal processing

Lecture - Searching the scientific literature

Chapter 22 - Basic statistical data analysis (lecture notes only)


Section B: Separations

Chapter 15 – Theory of molecular separations

Chapter 15 – Normal- and Reversed-phase liquid chromatography (bioanalysis)

Chapter 16 – Gas chromatography (environmental analysis)

Chapter 17 – Capillary electrophoresis (single cell analysis)


Section C: Spectroscopy

Chapter 7 – Atomic absorption spectroscopy

Chapter 9 – Atomic emission spectroscopy – ICP (water quality analysis)

Chapter 6 – Molecular UV/Vis spectroscopy

Chapter 8 – Molecular luminescence spectroscopy (bioanalysis)

Chapter 11 – Infrared spectroscopy (tissue imaging)

Chapter 12 – Raman spectroscopy (polymer characterization)


Section D: Electroanalytical Chemistry

Chapter 18 –  Potentiometry and probes (biomedical analysis)

Chapter 19 – Analytical voltammetry (chemical sensing)


Section E: Additional Topics

Chapter 20 - Material and surface analysis techniques (polymers and nanoscale materials)

Chapter 13 – Advanced topics in MS: understanding the experiment (biomolecules)





Exam Schedule

October 13th            (Exam 1 – in class)

November 10th        (Exam 2 – in class)

November 29th        (Exam 3 – in class)

December 15th         (Final Exam – 10:00-12:00 PM, Room 136)



There are a total of 800 points available for this course:


Ten weekly quizzes   Given during Recitation

(10 pts. each, 100 points total)

Three 1-h exams worth 100 points each

(300 points total)

Final Exam  Comprehensive

(200 points total)

Term Paper

(100 points total)

Oral presentation

(100 points total)


Religious Observances/ Other Absences from Class

It is the responsibility of students who plan to be absent from class at certain times throughout the semester, due to religious holidays or other reasons, to make arrangements in advance with the instructor. Course notes or handouts may be obtained from the instructor if these conditions are met. If a make-up exam is required, the instructor retains the right to determine the content of the exam and the conditions of administration, giving due consideration to equitable treatment.



Academic Honesty

Academic dishonesty at Michigan State University is defined by the General Student Regulations as conduct that violates the fundamental principles of truth, honesty, and integrity. The following conduct is specifically cited:


o    Supplying or using work or answers that are not one's own.

o   Providing or accepting assistance with completing assignments or examinations.

o    Interfering through any means with another's academic work.

o    Faking data or results.


You are expected to complete all course assignments, including homework, quizzes, tests and exams, without assistance from any source. You are expected to develop original work for this course; therefore, you may not submit course work you completed for another course to satisfy the requirements for this course.  Also, you are not authorized to use the or similar web sites to complete any course work in this course.


Students who violate these rules WILL be assigned a failing grade for the course.



Social Media Policy

As members of a learning community, students are expected to respect the intellectual property of course instructors. All course materials presented to students are the copyrighted property of the course instructor and are subject to the following conditions of use:


1)  Students may record lectures or any other classroom activities and use the recordings for their own course-related purposes only with permission from the instructor.


2)  If granted permission, students may share the recordings with other students enrolled in the class. Sharing is limited to using the recordings only for their own course-related purposes.


3)  Students may not post the recordings or other course materials online or distribute them to anyone not enrolled in the class without the advance written permission of the course instructor and, if applicable, any students whose voice or image is included in the recordings.


4)  Any student violating the conditions described above may face academic disciplinary sanctions.





Special Requests


Michigan State University is committed to providing equal opportunity for participation in all programs, services and activities. Requests for accommodations by persons with disabilities may be made by contacting the Resource Center for Persons with Disabilities at 517-884-RCPD or on the web at the link shown above. Once your eligibility for an accommodation has been determined, you will be issued a verified individual services accommodation (“VISA”) form. Please present this form to me at the start of the term and/or two weeks prior to the accommodation date (first test date). Requests received after this date will be honored whenever possible.