Chemistry
835 Fall 2017
Advanced
Analytical Chemistry II
Course Organization, Lecture Syllabus and Other Important Information
Chemistry 835 is the second of two courses on Advanced Analytical Chemistry. The course covers the subject areas of Molecular Spectroscopy, Mass Spectrometry, and Separation Science.
Textbook: The textbook, which is not used heavily, is Chemical Instrumentation: A Systematic Approach. Third Edition, by Howard A. Strobel and William R. Heineman, John Wiley & Sons, 1989. However, this text will not cover many concepts in the course and will be supplemented by other materials. If you do not wish to purchase this text, that is fine as older students may have a copy you can borrow. During the Mass Spectrometry module, students may find the following texts useful as references (but are not required reading): De Hoffmann, E. and Stroobant, V. "Mass Spectrometry: Principles and Applications", 3rd Edition, Wiley-Interscience, New York, 2007; Watson, J.T. and Sparkman, O.D. “Introduction to Mass Spectrometry: Instrumentation, Applications, and Strategies for Data Interpretation”, 4th Edition, Wiley, 2007.
Lectures: The lectures are given Monday, Wednesday, and Friday from 8:00 to 8:50 AM in Room 183 Chemistry. A tentative lecture schedule is attached. The lecturers are:
Professor Gary Blanchard (Molecular Spectroscopy)
Room 328 Chemistry
Phone: 353-1105
Email: blanchard@chemistry.msu.edu
Office Hours: Wednesday, 9 to 10 AM or by appointment
Professor Liangliang Sun (Mass Spectrometry and Separations Science)
Room 229 Chemistry
Phone: 353-0498
Email: lsun@chemistry.msu.edu
Office Hours: Wednesday, 9 to 10 AM or by appointment
Problem Sets: Problem sets will be given to aid in illustrating the concepts and principles discussed in the lectures. All students are required to do the problem sets because the ability to work these problems is often related to performance on the examinations, and is vital for understanding concepts. (Homework)
Quizzes: Frequent
quizzes may be given in class.
Examinations: Three 50-minute examinations will be given in the course. The dates of these exams are:
Hour Exam I: Monday, October 2nd, in class
Hour Exam II: Friday, October 27th, in class
Hour Exam III: Friday, December 8th, in class
Grading: The grading of the course will be on the following basis:
|
|
Percentage of total grade |
|
Molecular Spectroscopy |
33.3% |
|
Separation Science |
33.3% |
|
Mass Spectrometry |
33.3% |
Within the different areas, grading will be as follows.
|
Molecular
Spectroscopy |
|
|
|
Percentage of total
grade |
|
Quizzes |
25% |
|
Homework |
25% |
|
Exam 1 |
50% |
Separation Science |
|||||||||||
|
|
Percentage of total
grade |
||||||||||
|
Quizzes |
25% |
||||||||||
|
Homework |
25% |
||||||||||
|
Exam III |
50% |
||||||||||
Lecture
Syllabus
|
Lecture |
Date |
Topic |
Lecturer |
|
Part 1 – Molecular Spectroscopy |
|||
|
1 |
August 30 |
Course Introduction |
Blanchard |
|
2 |
September 1 |
Survey of spectroscopy, Beer’s Law |
Blanchard |
|
|
September 4 |
No Lecture – Labor
Day |
|
|
3 |
September 6 |
Interaction of light with matter I |
Blanchard |
|
4 |
September 8 |
Interaction of light with matter II |
Blanchard |
|
5 |
September 11 |
Optical susceptibility of materials |
Blanchard |
|
6 |
September 13 |
The Einstein coefficients |
Blanchard |
|
7 |
September 15 |
Molecular electronic and vibrational transitions |
Blanchard |
|
8 |
September 18 |
The Franck-Condon principle |
Blanchard |
|
9 |
September 20 |
Spontaneous Raman scattering |
Blanchard |
|
10 |
September 22 |
Linear and nonlinear response |
Blanchard |
|
11 |
September 25 |
Components 1 – choosing a light
source |
Blanchard |
|
12 |
September 27 |
Components 2 – wavelength
separation |
Blanchard |
|
13 |
September 29 |
Components 3 – detection
technology |
Blanchard |
|
|
October 2 |
Exam I |
Blanchard |
|
Part 2 – Mass Spectrometry (MS) |
|||
|
14 |
October 4 |
Introduction: importance of mass spectrometry; Information
in molecular ions (Mass and isotopes) |
Sun |
|
15 |
October 6 |
Ionization: Electron Ionization (EI),
Photoionization, and Chemical
ionization (CI) |
Sun |
|
16 |
October 9 |
Ionization:
Electrospray ionization (ESI) |
Sun |
|
17 |
October 11 |
Ionization: Matrix
Assisted Laser Desorption Ionization (MALDI) |
Sun |
|
18 |
October 13 |
Ionization: Ambient ionization techniques |
Sun |
|
19 |
October 16 |
Mass analyzer:
Filtering Mass analyzers (Sectors and Quadrupoles) |
Sun |
|
20 |
October 18 |
Mass analyzer: Ion
Trapping Mass analyzers (Ion Trap, Orbitrap and FT-ICR) |
Sun |
|
21 |
October 20 |
Visit Mass Spectrometry Facilities |
Sun |
|
22 |
October 23 |
Mass analyzer (Time-of-Flight
(TOF)), Detector and Vacuum |
Sun |
|
23 |
October 25 |
Tandem mass
spectrometry and Review |
Sun |
|
|
October 27 |
Exam II |
Sun |
|
Part 3 – Separation Science |
|||
|
24 |
October 30 |
Introduction: Importance
of separation; Resolution, Peak capacity, and Number of theoretical plates |
Sun |
|
25 |
November 1 |
Liquid
chromatography (LC): theory (separation mechanism) |
Sun |
|
26 |
November 3 |
LC: theory (The
Van Deemter equation) |
Sun |
|
27 |
November 6 |
LC: Reversed-Phase LC (RPLC) |
Sun |
|
28 |
November 8 |
LC: Ion Exchange
(IEX) and Size Exclusion Chromatography (SEC) |
Sun |
|
29 |
November 10 |
LC: Hydrophobic
Interaction Chromatography (HIC) and Hydrophilic Interaction Chromatography
(HILIC) |
Sun |
|
30 |
November 13 |
LC:
Multi-dimensional LC |
Sun |
|
31 |
November 15 |
Capillary
Electrophoresis (CE): Theory |
Sun |
|
32 |
November 17 |
Capillary Zone Electrophoresis (CZE) and Capillary Isoelectric
Focusing (cIEF) |
Sun |
|
33 |
November 20 |
Micellar Electrokinetic Chromatography (MEKC) and Capillary Gel Electrophoresis
(CGE) |
Sun |
|
34 |
November 22 |
Capillary
Isotachophoresis (cITP) |
Sun |
|
|
November 24 |
No Lecture - Thanksgiving |
Sun |
|
35 |
November 27 |
Microfluidic Chip and Gas Chromatography |
Sun |
|
36 |
November 29 |
Other Separation
Techniques and Detectors |
Sun |
|
37 |
December 1 |
Coupling Separation
to MS for Proteomics (I) |
Sun |
|
38 |
December 4 |
Coupling Separation to MS for Proteomics (II) |
Sun |
|
39 |
December 6 |
Review |
Sun |
|
|
December 8 |
Exam III |
Sun |
ADA Compliance
Michigan State University is committed to providing equal opportunity for participation in all programs, services, and activities. Requests for accommodations by persons with disabilities may be made by contacting the Resource Center for Persons with Disabilities by phone at 517-884-RCPD or through the web at rcpd.msu.edu. Once your eligibility for an accommodation has been determined, you will be issued a verified individual services accommodation (“VISA”) form. Please present this form to Professors Blanchard or Sun at the start of the semester and/or at least two weeks prior to the accommodation date (test, final exam, homework, etc.). Requests received after this date will be honored whenever possible.
Religious Observances /
Other Absences from Class:
It is the responsibility of students who plan to be absent from class at certain times throughout the semester, due to religious holidays or other reasons, to make arrangements in advance with the instructor. Course notes or handouts may be obtained from the instructor if these conditions are met. If a make-up exam is required, the instructor retains the right to determine the content of the exam and the conditions of administration, giving due consideration to equitable treatment.
Academic Honesty:
Academic dishonesty at Michigan State University is defined by the General Student Regulations as conduct that violates the fundamental principles of truth, honesty, and integrity. The following conduct is specifically cited:
Cheating - Providing or accepting assistance with completing assignments or examinations, without proper authorization.
Plagiarism - Supplying or using work or answers that are not one's own, without proper citation.
Fabrication- Faking data or results.
Sabotage- Interfering through any means with another's academic work.
Deception- Providing false information - e.g., giving a false excuse for missing a deadline or falsely claiming to have submitted work.
Students share with the faculty a responsibility for maintaining the integrity of scholarship, grades, and professional standards. CEM 835 adheres to the policies on academic honesty specified in General Student Regulation 1.0, Protection of Scholarship and Grades; the all-University Policy on Integrity of Scholarship and Grades; and Ordinance 17.00, Examinations. (See Spartan Life: Student Handbook and Resource Guide and/or the MSU Web site www.msu.edu.)
Unless authorized by your instructor, you are expected to complete all course assignments, including homework, lab work, quizzes, tests and exams, without assistance from any source. You are expected to develop original work for this course; therefore, you may not submit course work you completed for another course to satisfy the requirements for this course. Also, you are not authorized to use the www.allmsu.com Web site or similar web sites to complete any course work in this course.
Students who violate these rules will be assigned a failing grade for
the course.